Cirsium ×sudae: a new interspecific hybrid between rare Alpine thistles

Ester Michálková 1 , Jakub Šmerda 1 , Aleš Knoll 2 & Petr Bureš 1

Affiliations

  1. Department of Botany and Zoology, Faculty of Science, Masaryk University, Kotlářská 2, CZ-611 37 Brno, Czech Republic
  2. Department of Animal Morphology, Physiology and Genetics & CEITEC MENDELU, Mendel University in Brno, Zemědělská 1, CZ-613 00 Brno, Czech Republic

Published: 19 December 2018 , https://doi.org/10.23855/preslia.2018.347

PDF Appendices

Abstract

In this study we describe a new nothospecies, Cirsium ×sudae Michálková et Bureš, a homoploid hybrid between two rare Alpine species, C. carniolicum and C. greimleri. Hybrid status was confirmed for four morphologically intermediate individuals, found in the Ennstal Alps, Austria (three of them were F1 hybrids and one a backcross with C. carniolicum). We used amplified fragment length polymorphism (AFLP) to confirm affiliation to the parental species and exclude the potential contribution of other sympatric species. In addition, we used flow cytometry to confirm the diploid status of this hybrid. The newly assessed genome size of this hybrid is 2C = 1.99±0.03 pg, and for C. carniolicum 2C = 2.03±0.04 pg.

Keywords

AFLP, Alps, Asteraceae, Compositae, Carduoidae, Cynareae, flow cytometry, genome size, homoploid hybridization, interspecific hybridization, thistle

How to cite

Michálková E., Šmerda J., Knoll A. & Bureš P. (2018) Cirsium ×sudae: a new interspecific hybrid between rare Alpine thistles. – Preslia 90: 347–365, https://doi.org/10.23855/preslia.2018.347