Experimental hybridization in the genus Hieracium s. str.: crosses between diploid taxa.

The few attempts to produce artificial hybrids in the genus Hieracium s. str. have usually failed due to the use of polyploid parental taxa reproducing via agamospermy. Presented here for the first time are data on artificial hybridization in Hieracium s. str. which may help in understanding the microevolutionary processes resulting in the great morphological and genetic diversity in this genus. Diploid, sexually reproducing species (H. alpinum, H. pojoritense, H. transsilvanicum and two stable morphological types of H. umbellatum – of a low altitude and a high mountain type) were used as parent plants in experimental crosses. In most cases true hybrids, with intermediate morphology, were obtained. All the hybrids tested were diploid and produced a high amount of stainable pollen (65–92%). Hybrid progeny resulting from one cross exhibited a large range of morphological variation due to the combination of alleles from unrelated parental species. The percentage of welldeveloped achenes per capitulum, in capitula with at least one well-developed achene, in hybrids, ranged from 1.9 to 12.5% after free or controlled pollination, with an average of 4–5% per capitulum. Similar results (1.9–12.1%) were obtained from triple-cross hybrids. However, most of the capitula of hybrid progeny (either F1 or triple) were completely sterile after free or controlled pollination. Sterility is probably caused by genome incompatibility of unrelated parental taxa belonging to different sections. In two crosses, where strictly allogamous diploid plants of H. umbellatum (both morphotypes) were used as mother plants and F1 hybrids as pollen donors, some matroclinal progeny were obtained. This is a further example of the previously reported mentor effect. Diploid hybrids may be involved as pollen donors in gene flow as they produce uniformly sized and viable pollen. They are probably substantially less important as seed parents.